dynamicSPECT/pythonScripts/analysis.py

#analysis functions for cardiacSPECT dynamic data analysis
#see clusterAnalysis.ipynb for details on usage

import os
import getData
import config
import subprocess
import SimpleITK
import numpy
import re


def calculateCenters(db,setup,nclass,nr):

    
    #path of scripts is ../scripts
    #one up
    baseDir=os.path.dirname(os.getcwd())
    #scriptPath
    scriptPath=os.path.join(baseDir,'scripts','generateCenters.sh')
    rows=getData.getPatients(db,setup)
    for r in rows:
        #download 
        code=config.getCode(r,setup)
        for nc in nclass:
            print('centers: {}/{},{}'.format(code,nc,nr))
            print(subprocess.run([scriptPath,code,str(nc),str(nr)], \
                             check=True, stdout=subprocess.PIPE).stdout)
            
def doAnalysis(db,setup,nclass,nr,mode):
    baseDir=os.path.dirname(os.getcwd())#one up
    #this is if IVF is inferred together with k1 fits
    #runScript='doAnalysis.sh'
    #this is if IVF is taken from previous cluster fits
    #runScript='doAnalysisIVF.sh'
    
    
    if mode=='global':
        runScript='doAnalysis.sh'
        analysisType=''
    if mode=='IVF':
        runScript='doAnalysisIVF.sh'
        analysisType='IVF_'
    
    try:
        print('Setting runScript to {}'.format(runScript))
    except NameError:
        print('Mode can be one of (global,IVF))')
        return
        
    #either doAnalysis or doAnalysisIVF.sh
    scriptPath=os.path.join(baseDir,'scripts',runScript)

    rows=getData.getPatients(db,setup)
    for r in rows:
        dataPath=config.getLocalDir(r,setup)
        code=config.getCode(r,setup)
        for nc in nclass:
            for rId in numpy.arange(nr):
                print('{} [{} {}/{}]'.format(code,nc,rId+1,nr))
                #this is a duplicate of path generated in fitCenters.m
                fName=os.path.join(dataPath,'{}_{}_{}_{}fitParFinal.txt'.format(code,nc,rId+1,analysisType))
                if os.path.isfile(fName):
                    print('Skipping; {} available.'.format(fName))
                    continue
                subprocess.run([scriptPath,code,str(nc),str(rId+1)],\
                         check=True, stdout=subprocess.PIPE)

def doPixelAnalysis(db,setup,sigma2,mode='IVF'):
    
    baseDir=os.path.dirname(os.getcwd())#one up
                
    #in global mode, IVF parameters are inferred together with fits to classes
    #this is essentially repeat of the above, except that classes are taken as
    #time-response curves from pixels in the sigma2-defined neighborhood of
    #target pixels
    if mode=='global':
        runScript='doPixelAnalysis.sh'
        analysisType=''
    #in IVF mode, the parameters of input function are taken from the cluster fit 
    #(doAnalysis above, with mode=general). The rest is the same as for global mode
    if mode=='IVF':
        runScript='doPixelIVFAnalysis.sh'
        analysisType='IVF_'
   
    #either doPixelAnalysis or doPixelAnalysisIVF.sh
    scriptPath=os.path.join(baseDir,'scripts',runScript)


    try:
        print('Setting runScript to {}'.format(runScript))
    except NameError:
        print('Mode can be one of (global,IVF))')
        return
    
    rows=getData.getPatients(db,setup)
    for r in rows:
        dataPath=config.getLocalDir(r,setup)
        code=config.getCode(r,setup)
        sName=os.path.join(dataPath,'{}_Segmentation.txt'.format(code))
        sFile=os.path.join(dataPath,sName)
        x=numpy.loadtxt(sFile)
        nc=x.shape[0]
        for s2 in sigma2:
            sigmaCode='{:.2f}'.format(s2)
            sigmaCode=re.sub('\.','p',sigmaCode)
            fName=os.path.join(dataPath,'{}_{}_{}_Pixel{}_fitParFinal.txt'.format(code,nc,sigmaCode,analysisType))
            if os.path.isfile(fName):
                print('Skipping; {} available.'.format(fName))
                continue
            subprocess.run([scriptPath,code,str(s2)], \
                             check=True, stdout=subprocess.PIPE)
            
            
def getIWeights(r,setup,nclass,realizationId,ic):
    locDir=config.getLocalDir(r,setup)
    code=config.getCode(r,setup)
    fname='{}_{}_{}_center{}_centerWeigth.nrrd'.\
            format(code,nclass,realizationId+1,ic+1)
    uFile=os.path.join(locDir,fname)
            
    imU=SimpleITK.ReadImage(uFile)
    return SimpleITK.GetArrayFromImage(imU)

def getGaussianWeight(nU,pt,sigma2,na):
    #gaussian weighted summation of surrounding pixels
    
    #find point closest to the target point
    idx=[int(x) for x in pt]
    #running offset        
    fidx=numpy.zeros(3)
    #half of the neighborhood
    na2=0.5*(na-1)
    
    fs=0
    fWeight=0
    for i in numpy.arange(na):
        fidx[0]=idx[0]+(i-na2)
        for j in numpy.arange(na):
            fidx[1]=idx[1]+(j-na2)
            for k in numpy.arange(na):
                fidx[2]=idx[2]+(k-na2)
                fidx=[int(x) for x in fidx]
                fd=numpy.array(fidx)-numpy.array(pt)
                dist2=numpy.sum(fd*fd)
                fw=numpy.exp(-0.5*dist2/sigma2);
                fs+=fw
                fWeight+=fw*nU[tuple(fidx)]
                #print('\t{}/{}: {}/{:.2f} {:.2g} {:.3g} {:.2g}'.format(idx,fidx,numpy.sqrt(dist2),fw,nU[tuple(fidx)],fs,fWeight))
    fWeight/=fs
    return fWeight
            


#gets weights by class for a particular realization and sigma2 averaging
def getWeights(db,r,setup,nclass,realizationId,sigma2,na):
    #for w1, classes are in 0 to nclass-1 space
    #na is the size of the neighborhood
    idFilter=config.getIdFilter(r,setup)
    visitFilter=config.getVisitFilter(r,setup)
    code=config.getCode(r,setup)
    rows=getData.getSegmentation(db,setup,[idFilter,visitFilter])
    pts={r['regionId']:[float(x) for x in [r['x'],r['y'],r['z']]] for r in rows}
    
    w={region:numpy.zeros(nclass) for region in pts}
    na2=0.5*(na-1)
    
    for c in numpy.arange(nclass):
        nU=getIWeights(r,setup,nclass,realizationId,c)
        
        for region in w:
            #print(pts[region])
            #print('{} {}/{} {}'.format(code,c,nclass,region))
            #gaussian weighted summation of surrounding pixels
            w[region][c]=getGaussianWeight(nU,pts[region],sigma2,na)
            
            
    return w


#gets fitPar for a particular realization in [0..nr-1] range
def getFitPar(r,setup,nclass,realizationId,mode=''):
    #fitGoodnes A tau alpha delay [k1 BVF k2 delay]xNcenters
    allowedModes=['','IVF','Pixel','PixelIVF']
    if mode not in allowedModes:
        print('Mode should be one of {}'.format(allowedModes))
        return []
    
    if mode=='PixelIVF':
        #4th parameter is sigma, not realizationId
        rCode='{:.2f}'.format(realizationId)
        rCode=re.sub('\.','p',rCode)
    else:
        #add one to match matlab 1..N array indexing
        rCode='{}'.format(realizationId+1)
    
    if len(mode)>0:
        mode=mode+'_'
    
    code=config.getCode(r,setup)
    fname='{}_{}_{}_{}fitParFinal.txt'.format(code,nclass,rCode,mode)
    locDir=config.getLocalDir(r,setup)
    uFile=os.path.join(locDir,fname)
    return numpy.genfromtxt(uFile,delimiter='\t')

def getK1(fitPar,iclass):
    #fitGoodnes A tau alpha delay [k1 BVF k2 delay]xNcenters
    #0 to nclass-1 space
    return fitPar[4*iclass+5]

def calculateK1(w,fitPar):
    #calculateK1 for region weights
    #return the k1 that belongs to the 
    #maximum class in region (M) and 
    #a weighted average (W)
    k1={region:{'M':0,'W':0} for region in w}
    for region in w:
        cmax=numpy.argmax(w[region])
        k1[region]['M']=getK1(fitPar,cmax)
        fs=0
        for c in numpy.arange(len(w[region])):
            fs+=w[region][c]*getK1(fitPar,c)
        k1[region]['W']=fs
    return k1

def getPatientValuesByNclass(db,r,setup,nclass,nrealizations,sigma2,na):
    #summary script
    #db is for database; needs segmentations
    #r,setup identify patient
    #nclass and nrealizations select strategy
    #sigma2 is for combining output from adjacent pixels
    #na is neighborhood size where smoothing/combination is done
    k1={}
    for rId in numpy.arange(nrealizations):
        w=getWeights(db,r,setup,nclass,rId,sigma2,na)
        fitPar=getFitPar(r,setup,nclass,rId,'IVF')
        qk1=calculateK1(w,fitPar)
        for region in w:
            for type in qk1[region]:
                try:
                    k1[region][type].append(qk1[region][type])
                except KeyError:
                    k1={region:{type:[] for type in qk1[region]} for region in w}
                    print(type)
                    k1[region][type].append(qk1[region][type])
        print('[{}] {}/{}'.format(nclass,rId+1,nrealizations))
    return k1   

def getSummaryPatientValuesByNclass(db,r,setup,nclass,nrealizations,sigma2,na):
    #summary script, same arguments as above
    #also return deviation over realization
    k1=getPatientValuesByNclass(db,r,setup,nclass,nrealizations,sigma2,na)
    avgType=['M','W']
    summaryK1={type:{region:{
        'mean':numpy.mean(k1[region][type]), 
        'std':numpy.std(k1[region][type]), 
        'median':numpy.median(k1[region][type])} for region in k1}
               for type in avgType}
    
    return summaryK1

def fullSummary(db,setup,classes,nr,sigma2,na):
    rows=getData.getPatients(db,setup)
    return \
        {config.getCode(r,setup):\
         {c:getSummaryPatientValuesByNclass(db,r,setup,c,nr,sigma2,na) for c in classes} for r in rows}
          
def storeSummary(db,setup,summary,sigma2,na):
    #dsM=db.selectRows(project,'study','Imaging',[])
    for rCode in summary:
        r=config.decode(rCode,setup)
        idFilter=config.getIdFilter(r,setup)
        visitFilter=config.getVisitFilter(r,setup)
        for c in summary[rCode]:
            cFilter={'variable':'nclass','value':str(c),'oper':'eq'}
            for t in summary[rCode][c]:
                tFilter={'variable':'option','value':t,'oper':'eq'}
                for rId in summary[rCode][c][t]:
                    rFilter={'variable':'regionId','value':str(rId),'oper':'eq'}
                    rows=getData.getSummary(db,setup,[idFilter,visitFilter,cFilter,tFilter,rFilter])
                    if len(rows)>0:
                        qrow=rows[0]
                        mode='update'
                    else:
                        qrow={qr:r[qr] for qr in r}
                        qrow['nclass']=c
                        qrow['option']=t
                        qrow['regionId']=rId
                        seqNum=config.getTargetSeqNum(r,setup)
                        qrow['SequenceNum']=100+seqNum+c+0.001*rId
                        if t=='M':
                            qrow['SequenceNum']+=0.0001
                        mode='insert'
                    for v in summary[rCode][c][t][rId]:
                        qrow[v]=summary[rCode][c][t][rId][v]
                    qrow['sigma2']=sigma2
                    qrow['na']=na
                    getData.updateSummary(db,setup,mode,[qrow])
                    
def summaryPixelIVF(db,setup,sigma2):
    #for second type of analysis (pixel based regions)
    rows=getData.getPatients(db,setup)
    return \
        {config.getCode(r,setup):\
         {s2:getPixelIVF(db,r,setup,s2) for s2 in sigma2} for r in rows}

    
def storeIVF(db,setup,summary):
    for rCode in summary:
        r=config.decode(rCode,setup)
        idFilter=config.getIdFilter(r,setup)
        visitFilter=config.getVisitFilter(r,setup)
        for s2 in summary[rCode]:
            sigmaFilter={'variable':'sigma2','value':str(s2),'oper':'eq'}
            nr=len(summary[rCode][s2])
            for rId in summary[rCode][s2]:
                rFilter={'variable':'regionId','value':str(rId),'oper':'eq'}
                typeFilter={'variable':'option','value':'D','oper':'eq'}
                rows=getData.getSummary(db,setup,[idFilter,visitFilter,sigmaFilter,rFilter,typeFilter])
                if len(rows)>0:
                    qrow=rows[0]
                    mode='update'
                else:
                    qrow={qr:r[qr] for qr in r}
                    qrow['sigma2']=s2
                    qrow['regionId']=rId
                    seqNum=config.getTargetSeqNum(r,setup)
                    qrow['SequenceNum']=140+seqNum+0.01*rId+0.001*s2
                    mode='insert'
                qrow['mean']=summary[rCode][s2][rId]
                qrow['na']=7
                qrow['nclass']=nr
                qrow['option']='D'
                getData.updateSummary(db,setup,mode,[qrow])
                
def getPixelIVF(db,r,setup,sigma2):
    idFilter=config.getIdFilter(r,setup)
    visitFilter=config.getVisitFilter(r,setup)
    rows=getData.getSegmentation(db,setup,[idFilter,visitFilter])
    nclassIVF=len(rows)
    fitPar=getFitPar(r,setup,nclassIVF,sigma2,'PixelIVF')
    k1={r['regionId']:getK1(fitPar,r['regionId']) for r in rows}
    return k1